Journal: bioRxiv

Article Title: It runs in the family: Discovery of enzymes in the oleuropein pathway in Olive ( Olea europaea ) by comparative transcriptomics

doi: 10.1101/2025.03.20.644408

Figure Lengend Snippet: Schematic of differentially expressed genes (DEG) belonging to enriched KEGG modules of terpenoid biosynthesis, increasing (M00095 – MVA pathway; top) or decreasing (M00096 – MEP pathway; center) during olive maturation and ripening. The secoiridoid biosynthetic pathway is shown at the bottom, with enzymes characterized in this work shown in olive green; ligstroside biosynthesis remains unsolved and thus it is represented as disconnected. Heatmaps show expression patterns of all DEGs (one per row) annotated as the KEGG orthology number shown below each plot, with colors showing the average of the log-transformed transcripts per million (log 2 (TPM+1)) of all species, with color-scale ranging from the minimum (blue) to the maximum (red) mean expression value per gene (row.) Scatter plots show the mean (dots) and standard deviation (bars) of the intensity, scaled by species, of standard-confirmed iridoids through the five measured stages of maturation. MVA pathway: ACAT, acetyl coenzyme A (CoA) acetyltransferase; HMGS, hydroxymethylglutaryl-CoA synthase; HMGR, hydroxymethylglutaryl-CoA reductase; MK, mevalonate kinase; PMK, phosphomevalonate kinase; MDD, mevalonate diphosphate decarboxylase; isopentenyl diphosphate delta-isomerase (IDI). MEP pathway: DXS, 1-deoxy-D-xylulose-5-phosphate synthase; DXR, 1-deoxy-D-xylulose-5-phosphate reductoisomerase;MCT, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; CMK, 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol kinase; MDS, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; HDS, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase; HMDR, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase; GPPS, geranyl pyrophosphate synthase. Iridoid biosynthesis: GES, geraniol synthase; G8H, geraniol 8-hydroxylase; 8HGO, 8-hydroxygeraniol oxidoreductase; ISY, iridoid synthase; ISP, iridoid synthase paralogue; IO, iridoid oxidase; 7DLGT, 7-deoxyloganetic acid glucosyltransferase; 7eLAS, 7- epi -loganic acid synthase; 7eLAMT, 7- epi -loganic acid O-methyltransferase; OMES, oleoside methyl ester synthase; OMEGT, oleoside-11-methyl ester glucosyl transferase; OS, oleuropein synthase.

Article Snippet: The rest of the iridoid standards (7-deoxy-loganic acid, 7-deoxy-8- epi -loganic acid, 7-deoxy-loganin, 7-deoxy-8- epi -loganic acid, 7- epi -loganic acid, 7- epi -loganin, and 7-ketologanin) were synthesized from commercially available geniposide ( Biosynth Carbosynth , Staad, Switzerland) as previously reported ( ).

Techniques: Expressing, Transformation Assay, Standard Deviation

Journal: Nanomaterials

Article Title: Incorporation of Lippia citriodora Microwave Extract into Total-Green Biogelatin-Phospholipid Vesicles to Improve Its Antioxidant Activity

doi: 10.3390/nano10040765

Figure Lengend Snippet: Qualitative and quantitative results of L. citriodora extract.

Article Snippet: The calibration curves were built by using available commercial standards of verbascoside, loganic acid, kaempferol-3-glucoside, quercetin and apigenin purchased from Extrasynthese (Genay Cedex, France), Fluka or Sigma Aldrich (Steinheim, Germany).

Techniques:

Journal: Journal of Analytical Methods in Chemistry

Article Title: Quantitative and Qualitative Characterization of Gentiana rigescens Franch (Gentianaceae) on Different Parts and Cultivations Years by HPLC and FTIR Spectroscopy

doi: 10.1155/2017/3194146

Figure Lengend Snippet: The calibration curves, r 2 , LOD, and LOQ of the standard compounds.

Article Snippet: The chemical standards of loganic acid, swertiamarin, gentiopicroside, and sweroside were provided by Shifeng Biological Technology Co., Ltd. (Shanghai, China), and Chinese National Institute for Food and Drug Control (Beijing, China).

Techniques: